Journal: Traffic (Copenhagen, Denmark)

Article Title: Simplified equation to extract diffusion coefficients from confocal FRAP data

doi: 10.1111/tra.12008

Figure Lengend Snippet: List of parameters and their definitions

Article Snippet: Data fitting was carried out for D and M f by a nonlinear least-squares fitting routine (nlinfit.m) available in MATLAB ® (version 7.10, R2010a, The Mathworks, Inc.) minimizing a weighted residual between averaged FRAP data from 10 experiments ( F Data ( t )) and a theoretical FRAP curve ( F ( t ), Eq.

Techniques: Diffusion-based Assay, Fluorescence, Sampling, Protein Concentration

Journal: Traffic (Copenhagen, Denmark)

Article Title: Simplified equation to extract diffusion coefficients from confocal FRAP data

doi: 10.1111/tra.12008

Figure Lengend Snippet: (A) Representative images of Alexa-CTxB on the plasma membrane during a FRAP experiment on either live or fixed cells for rn=1.1μm. Scale bar = 1 μm. (B) Postbleach profiles of Alexa-CTxB on the plasma membranes of live (□, n=12) and fixed (○, n=5) cells. (C) FRAP data of Alexa-CTxB on the plasma membranes of live (□, n=12) and fixed (○, n=5) cells. Since the bleach ROI is slightly off center in our system as seen in the images of (A) at t=0, a correction was made to align the center of the postbleach profile to determine re.

Article Snippet: Data fitting was carried out for D and M f by a nonlinear least-squares fitting routine (nlinfit.m) available in MATLAB ® (version 7.10, R2010a, The Mathworks, Inc.) minimizing a weighted residual between averaged FRAP data from 10 experiments ( F Data ( t )) and a theoretical FRAP curve ( F ( t ), Eq.

Techniques:

Journal: Traffic (Copenhagen, Denmark)

Article Title: Simplified equation to extract diffusion coefficients from confocal FRAP data

doi: 10.1111/tra.12008

Figure Lengend Snippet: Comparison of diffusion coefficients determined by FRAP data fitting (DFitting, Eq. 3), versus the DConfocal equation (Eq. 5), or the Soumpasis equation using either rn (Drn) or re (Dre) in log scale. D’s were found from averaged FRAP curves (N≥12 cells per experiment) for three or more separate experiments (n≥3). Error bars represent standard errors. Dashed boxes show D’s reported in the literature (Table 3). *, p<0.05 compared to DFitting, Student’s t-test.

Article Snippet: Data fitting was carried out for D and M f by a nonlinear least-squares fitting routine (nlinfit.m) available in MATLAB ® (version 7.10, R2010a, The Mathworks, Inc.) minimizing a weighted residual between averaged FRAP data from 10 experiments ( F Data ( t )) and a theoretical FRAP curve ( F ( t ), Eq.

Techniques: Diffusion-based Assay

Journal: Traffic (Copenhagen, Denmark)

Article Title: Simplified equation to extract diffusion coefficients from confocal FRAP data

doi: 10.1111/tra.12008

Figure Lengend Snippet: Diffusion coefficients were determined by FRAP data fitting (DFitting, Eq. 3), by the DConfocal equation (Eq. 5), by the Soumpasis equation using rn (Drn), and by the Soumpasis equation using re (Dre) for individual confocal FRAP curves (N≥6). Dashed box indicates the range of EGFP’s diffusion coefficients in the cytosol reported in the literature. re was measured from an averaged postbleach profile (n=1,N=10 cells) and D’s were obtained from individual FRAP data (n=1, N=8,10,10, and 8 cells). *, p<0.05 compared to DFitting, Student’s t-test.

Article Snippet: Data fitting was carried out for D and M f by a nonlinear least-squares fitting routine (nlinfit.m) available in MATLAB ® (version 7.10, R2010a, The Mathworks, Inc.) minimizing a weighted residual between averaged FRAP data from 10 experiments ( F Data ( t )) and a theoretical FRAP curve ( F ( t ), Eq.

Techniques: Diffusion-based Assay

Journal: Traffic (Copenhagen, Denmark)

Article Title: Simplified equation to extract diffusion coefficients from confocal FRAP data

doi: 10.1111/tra.12008

Figure Lengend Snippet: Comparison of Mean±SE of (A) re, (B) τ1/2, (C) D, and (D) Mf determined using averaged FRAP data from more than three independent experiements with 10 cells (n≥3, N=10) or means from 10 individual FRAP data in a single experiment (N=10). Error bars represent standard errors. p>0.05 Cross comparison, Student’s t-test.

Article Snippet: Data fitting was carried out for D and M f by a nonlinear least-squares fitting routine (nlinfit.m) available in MATLAB ® (version 7.10, R2010a, The Mathworks, Inc.) minimizing a weighted residual between averaged FRAP data from 10 experiments ( F Data ( t )) and a theoretical FRAP curve ( F ( t ), Eq.

Techniques: